In 2001, Hagan Bayley’s lab at Texas A&M demonstrated a limited sequencing method based on the observation that correctly and incorrectly paired DNA bases disrupted nanopore current to different extents. They tethered a short piece of DNA with a few unknown bases to the entrance of the nanopore, then added other short DNA strands with different bases at the position corresponding to the unknown base on the tethered strand. By looking at which base produced the disruption corresponding to a perfect match, they could guess the unknown nucleotide.
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